Mitochondria Isolated from Rat Brown Adipose Tissue and Liver

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Mitochondria Isolated from Rat Brown Adipose Tissue and Liver

Mitochondrial fractions, relatively free from contamination by other cytoplasmic structures, have been isolated by differential centrifugation from homogenates of brown adipose tissue from starved rats. It was possible in such fractions to distinguish two types of mitochondria in this tissue. Type I mitochondria, when morphologically intact, are limited by a bilaminar membrane and show regular ...

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Mitochondria Isolated from Rat Brown Adipose Tissue

Mitochondrial fractions, relatively free from contsmination by other cytopla-~mic structures, have been isolated by differential centrifugation from homogenates of brown adipose tissue from starved rats. It was possible in such fractions to distinguish two types of mitochondria in this tissue. Type I mitochondria, when morphologically intact, are limited by a bilaminar membrane and show regular...

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Mitochondria Isolated from Rat Brown Adipose Tissue and BY J. D. LEVER, M~D, AND J. B. CHAPPELL, PH.D

Mitochondrial fractions, relatively free from contsmination by other cytopla-~mic structures, have been isolated by differential centrifugation from homogenates of brown adipose tissue from starved rats. It was possible in such fractions to distinguish two types of mitochondria in this tissue. Type I mitochondria, when morphologically intact, are limited by a bilaminar membrane and show regular...

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Sodium-induced calcium release from mitochondria in brown adipose tissue.

Coupled mitochondria of brown adipose tissue can accumulate Ca2+ if a substrate is present. The Ca2+ is released by addition of 20 mM Na+, but not by addition of K+ or choline +. Energy-dissipating Na+-induced Ca2+ cycling occurs maximally with 20 mM Na+ and 10 microM Ca2+. In brown adipocytes, the Ca2+ ionophore A23187 and the Na+ ionophore monensin increase respiration if substrate is added, ...

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ژورنال

عنوان ژورنال: The Journal of Biophysical and Biochemical Cytology

سال: 1958

ISSN: 1540-8140,0021-9525

DOI: 10.1083/jcb.4.3.287